Conserved CA-rich motifs in gene promoters of Pt x tMYB021-responsive secondary cell wall carbohydrate-active enzymes in Populus.
Identifieur interne : 003321 ( Main/Exploration ); précédent : 003320; suivant : 003322Conserved CA-rich motifs in gene promoters of Pt x tMYB021-responsive secondary cell wall carbohydrate-active enzymes in Populus.
Auteurs : Anders Winzell [Suède] ; Henrik Aspeborg ; Yucheng Wang ; Ines EzcurraSource :
- Biochemical and biophysical research communications [ 1090-2104 ] ; 2010.
Descripteurs français
- KwdFr :
- Arabidopsis (génétique), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Facteurs de transcription (génétique), Métabolisme glucidique (MeSH), Paroi cellulaire (enzymologie), Populus (croissance et développement), Populus (enzymologie), Populus (génétique), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Séquence conservée (MeSH), Séquence nucléotidique (MeSH), Xylème (croissance et développement), Xylème (enzymologie), Xylème (génétique).
- MESH :
- croissance et développement : Populus, Xylème.
- enzymologie : Paroi cellulaire, Populus, Xylème.
- génétique : Arabidopsis, Facteurs de transcription, Populus, Xylème.
- Clonage moléculaire, Données de séquences moléculaires, Métabolisme glucidique, Régions promotrices (génétique), Régulation de l'expression des gènes végétaux, Séquence conservée, Séquence nucléotidique.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Base Sequence (MeSH), Carbohydrate Metabolism (MeSH), Cell Wall (enzymology), Cloning, Molecular (MeSH), Conserved Sequence (MeSH), Gene Expression Regulation, Plant (MeSH), Molecular Sequence Data (MeSH), Populus (enzymology), Populus (genetics), Populus (growth & development), Promoter Regions, Genetic (MeSH), Transcription Factors (genetics), Xylem (enzymology), Xylem (genetics), Xylem (growth & development).
- MESH :
- chemical , genetics : Transcription Factors.
- enzymology : Cell Wall, Populus, Xylem.
- genetics : Arabidopsis, Populus, Xylem.
- growth & development : Populus, Xylem.
- Base Sequence, Carbohydrate Metabolism, Cloning, Molecular, Conserved Sequence, Gene Expression Regulation, Plant, Molecular Sequence Data, Promoter Regions, Genetic.
Abstract
In order to understand gene regulation during wood formation, we cloned a MYB46-like gene in hybrid aspen, Populus tremula x tremuloides, called Pt x tMYB021. Phylogenetic and paired identity analysis of MYB46-like genes in Populus and Arabidopsis reveals relationships between paralogous pairs of Populus MYB46-like proteins and their Arabidopsis counterparts MYB46 and MYB83, and suggest that Pt x tMYB021 is the ortholog of MYB46. Pt x tMYB021 is expressed mainly in xylem tissues, and transiently expressed Pt x tMYB46 transactivates gene promoters of xylan-active CAZymes GT43A, GT43B and Xyn10A. Analysis of conserved motifs within these promoters identify the sequence CCACCAAC, called ACTYP, which is similar to the AC elements mediating transactivation by MYB transcription factors during lignin biosynthesis. Further analysis by Motif Finder identifies four 6 bp CA-rich motifs overlapping ACTYP, and we show that these motifs are enriched in xylem-specific promoters. We propose that AC-type regulatory elements mediate xylem-specific MYB46-dependent expression of secondary cell wall carbohydrate-active enzymes (CAZymes), besides activating gene expression of lignin biosynthesis enzymes.
DOI: 10.1016/j.bbrc.2010.03.101
PubMed: 20307500
Affiliations:
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first="Yucheng" last="Wang">Yucheng Wang</name></author><author><name sortKey="Ezcurra, Ines" sort="Ezcurra, Ines" uniqKey="Ezcurra I" first="Ines" last="Ezcurra">Ines Ezcurra</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20307500</idno><idno type="pmid">20307500</idno><idno type="doi">10.1016/j.bbrc.2010.03.101</idno><idno type="wicri:Area/Main/Corpus">003257</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003257</idno><idno type="wicri:Area/Main/Curation">003257</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003257</idno><idno type="wicri:Area/Main/Exploration">003257</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Conserved CA-rich motifs in gene promoters of Pt x tMYB021-responsive secondary cell wall carbohydrate-active enzymes in Populus.</title><author><name sortKey="Winzell, Anders" sort="Winzell, Anders" uniqKey="Winzell A" first="Anders" last="Winzell">Anders Winzell</name><affiliation wicri:level="1"><nlm:affiliation>Royal Institute of Technology, Department of Biotechnology, AlbaNova University Center, SE-10691 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Royal Institute of Technology, Department of Biotechnology, AlbaNova University Center, SE-10691 Stockholm</wicri:regionArea><wicri:noRegion>SE-10691 Stockholm</wicri:noRegion></affiliation></author><author><name sortKey="Aspeborg, Henrik" sort="Aspeborg, Henrik" uniqKey="Aspeborg H" first="Henrik" last="Aspeborg">Henrik Aspeborg</name></author><author><name sortKey="Wang, Yucheng" sort="Wang, Yucheng" uniqKey="Wang Y" first="Yucheng" last="Wang">Yucheng Wang</name></author><author><name sortKey="Ezcurra, Ines" sort="Ezcurra, Ines" uniqKey="Ezcurra I" first="Ines" last="Ezcurra">Ines Ezcurra</name></author></analytic><series><title level="j">Biochemical 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(MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Facteurs de transcription (génétique)</term><term>Métabolisme glucidique (MeSH)</term><term>Paroi cellulaire (enzymologie)</term><term>Populus (croissance et développement)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Séquence conservée (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Xylème (croissance et développement)</term><term>Xylème (enzymologie)</term><term>Xylème (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Paroi cellulaire</term><term>Populus</term><term>Xylème</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cell Wall</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arabidopsis</term><term>Facteurs de transcription</term><term>Populus</term><term>Xylème</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Carbohydrate Metabolism</term><term>Cloning, Molecular</term><term>Conserved Sequence</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Métabolisme glucidique</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term><term>Séquence conservée</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to understand gene regulation during wood formation, we cloned a MYB46-like gene in hybrid aspen, Populus tremula x tremuloides, called Pt x tMYB021. Phylogenetic and paired identity analysis of MYB46-like genes in Populus and Arabidopsis reveals relationships between paralogous pairs of Populus MYB46-like proteins and their Arabidopsis counterparts MYB46 and MYB83, and suggest that Pt x tMYB021 is the ortholog of MYB46. Pt x tMYB021 is expressed mainly in xylem tissues, and transiently expressed Pt x tMYB46 transactivates gene promoters of xylan-active CAZymes GT43A, GT43B and Xyn10A. Analysis of conserved motifs within these promoters identify the sequence CCACCAAC, called ACTYP, which is similar to the AC elements mediating transactivation by MYB transcription factors during lignin biosynthesis. Further analysis by Motif Finder identifies four 6 bp CA-rich motifs overlapping ACTYP, and we show that these motifs are enriched in xylem-specific promoters. We propose that AC-type regulatory elements mediate xylem-specific MYB46-dependent expression of secondary cell wall carbohydrate-active enzymes (CAZymes), besides activating gene expression of lignin biosynthesis enzymes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20307500</PMID><DateCompleted><Year>2010</Year><Month>05</Month><Day>04</Day></DateCompleted><DateRevised><Year>2010</Year><Month>04</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2104</ISSN><JournalIssue CitedMedium="Internet"><Volume>394</Volume><Issue>3</Issue><PubDate><Year>2010</Year><Month>Apr</Month><Day>09</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem Biophys Res Commun</ISOAbbreviation></Journal><ArticleTitle>Conserved CA-rich motifs in gene promoters of Pt x tMYB021-responsive secondary cell wall carbohydrate-active enzymes in Populus.</ArticleTitle><Pagination><MedlinePgn>848-53</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bbrc.2010.03.101</ELocationID><Abstract><AbstractText>In order to understand gene regulation during wood formation, we cloned a MYB46-like gene in hybrid aspen, Populus tremula x tremuloides, called Pt x tMYB021. Phylogenetic and paired identity analysis of MYB46-like genes in Populus and Arabidopsis reveals relationships between paralogous pairs of Populus MYB46-like proteins and their Arabidopsis counterparts MYB46 and MYB83, and suggest that Pt x tMYB021 is the ortholog of MYB46. Pt x tMYB021 is expressed mainly in xylem tissues, and transiently expressed Pt x tMYB46 transactivates gene promoters of xylan-active CAZymes GT43A, GT43B and Xyn10A. Analysis of conserved motifs within these promoters identify the sequence CCACCAAC, called ACTYP, which is similar to the AC elements mediating transactivation by MYB transcription factors during lignin biosynthesis. Further analysis by Motif Finder identifies four 6 bp CA-rich motifs overlapping ACTYP, and we show that these motifs are enriched in xylem-specific promoters. We propose that AC-type regulatory elements mediate xylem-specific MYB46-dependent expression of secondary cell wall carbohydrate-active enzymes (CAZymes), besides activating gene expression of lignin biosynthesis enzymes.</AbstractText><CopyrightInformation>Copyright 2010 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Winzell</LastName><ForeName>Anders</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Royal Institute of Technology, Department of Biotechnology, AlbaNova University Center, SE-10691 Stockholm, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aspeborg</LastName><ForeName>Henrik</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yucheng</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Ezcurra</LastName><ForeName>Ines</ForeName><Initials>I</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>03</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="Y">Conserved Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>03</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>03</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>3</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>3</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>5</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20307500</ArticleId><ArticleId IdType="pii">S0006-291X(10)00565-6</ArticleId><ArticleId IdType="doi">10.1016/j.bbrc.2010.03.101</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Aspeborg, Henrik" sort="Aspeborg, Henrik" uniqKey="Aspeborg H" first="Henrik" last="Aspeborg">Henrik Aspeborg</name><name sortKey="Ezcurra, Ines" sort="Ezcurra, Ines" uniqKey="Ezcurra I" first="Ines" last="Ezcurra">Ines Ezcurra</name><name sortKey="Wang, Yucheng" sort="Wang, Yucheng" uniqKey="Wang Y" first="Yucheng" last="Wang">Yucheng Wang</name></noCountry><country name="Suède"><noRegion><name sortKey="Winzell, Anders" sort="Winzell, Anders" uniqKey="Winzell A" first="Anders" last="Winzell">Anders Winzell</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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